glial cell line svgp12  (ATCC)

Journal: Nutrients

Article Title: Methanolic Extract of Cimicifuga foetida Induces G 1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells

doi: 10.3390/nu16193254

Figure Lengend Snippet: Effects of water (CF-WE) and methanol (CF-ME) extracts of Cimicifuga foetida on the viability of glioblastoma (GBM) and glial cells. ( A ) Cell viability of U87 MG, A172, and T98G GBM cell lines treated with varying concentrations (0–800 μg/mL) of CF-WE for 24, 48, and 72 h showed a significant dose- and time-dependent decrease in cell viability, with stronger effects at higher concentrations and longer treatment durations. ( B ) Cell viability of U87 MG, A172, and T98G GBM cell lines treated with CF-ME (0–150 μg/mL) for the same durations exhibited a stronger inhibitory effect, also in a dose- and time-dependent manner. ( C ) Cell viability of human normal glial cell line SVGp12 treated with CF-WE (left) and CF-ME (right) under similar conditions showed reduced cell viability, but SVGp12 cells exhibited higher overall viability and IC 50 values than GBM cells, indicating lower toxicity toward normal glial cells. * Asterisks indicate statistical significance compared to control (0 μg/mL) ( p < 0.05). Error bars represent the mean ± SD of three independent experiments.

Article Snippet: U87 MG, A172, and T98G (GBM cell lines) and SVGp12 (normal human glial cell line) were obtained from the Bioresource Collection and Research Center (BCRC).

Techniques: Control

Journal: Nutrients

Article Title: Methanolic Extract of Cimicifuga foetida Induces G 1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells

doi: 10.3390/nu16193254

Figure Lengend Snippet: IC 50 values of malignant glioma cells and normal glial cells after treatment with extract of Cimicifuga foetida . U87 MG, A172, T98G, and SVGp12 cells were treated with different doses of CF-WE and CF-ME for 72 h, respectively. Cell viability was detected using an MTT assay with an ELISA reader to measure absorbance values. IC 50 values were calculated after plotting the regression lines. Data are presented as means ± standard deviation.

Article Snippet: U87 MG, A172, and T98G (GBM cell lines) and SVGp12 (normal human glial cell line) were obtained from the Bioresource Collection and Research Center (BCRC).

Techniques: MTT Assay, Enzyme-linked Immunosorbent Assay

Journal: Molecules

Article Title: Andrographolide Induces G2/M Cell Cycle Arrest and Apoptosis in Human Glioblastoma DBTRG-05MG Cell Line via ERK1/2 /c-Myc/p53 Signaling Pathway

doi: 10.3390/molecules27196686

Figure Lengend Snippet: The percentage of cell viability of ( A ) DBTRG-05MG, ( B ) SVGp12 treated with andrographolide, ( C ) DBTRG-05MG treated with temozolomide in a time frame of 24, 48, and 72 h. The results were analyzed in GraphPad Prism 8.0.1 (Graphpad software, Inc., San Diego, CA, USA). Data expressed as mean ± SEM and statistical analysis between three independent groups was performed by t-test compared with the untreated group: * p < 0.05, ** p < 0.005, *** p < 0.0005.

Article Snippet: Human glioblastoma cell lines DBTRG-05MG and normal glial cell lines SVGp12 were supplied by the American Type Culture Collection (ATCC), USA.

Techniques: Software

Journal: Molecules

Article Title: Andrographolide Induces G2/M Cell Cycle Arrest and Apoptosis in Human Glioblastoma DBTRG-05MG Cell Line via ERK1/2 /c-Myc/p53 Signaling Pathway

doi: 10.3390/molecules27196686

Figure Lengend Snippet: The half-maximal lethal concentration (LC 50 ) values for ( A ) DBTRG-05MG and SVGp12 cells after being treated with andrographolide (0.781–200 µM) and ( B ) DBTRG-05MG cells after being treated with temozolomide (0.781–200 µM) were estimated using nonlinear regression approximations in GraphPad Prism 8.0.1 (Graphpad software, Inc., San Diego, CA, USA).

Article Snippet: Human glioblastoma cell lines DBTRG-05MG and normal glial cell lines SVGp12 were supplied by the American Type Culture Collection (ATCC), USA.

Techniques: Concentration Assay, Software